Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies
Fig 2
Detection limits of LAMP assays.
(A-C) TP LAMP assay involving (A) TPA strain Nichols with copy numbers 10E+5 to 10E+0, (B) TPE strain Gauthier tested in a range of 10E+6 to 10E+0 copies, and (C) TEN strain Bosnia A in 10E+6 to 10E+0 copy numbers. (D-E) TPA LAMP assay run with TPA strain Nichols from 10E+5 to 10E+0 copy numbers, and the same LAMP assay run with (E) TEN strain Bosnia A in copy numbers ranging from 10E+6 to 10E+0. (F-G) TPE/TEN LAMP assay involving (E) TPE strain Gauthier tested in 10E+6 to 10E+0 copy numbers, and (F) TEN strain Bosnia A tested in 10E+6 to 10E+0 copies. Red cross = Negative control, TPA strain Nichols (blue), TPE strain Gauthier (green), TEN strain Bosnia A (black); symbols represent copy numbers: circle with cross = 10E+6, hexagon = 10E+5, square turned = 10E+4, square = 10E+3, circle = 10E+2, down-pointing triangle = 10E+1, up-pointing triangle = 10E+0.
