Aedes cadherin receptor that mediates Bacillus thuringiensis Cry11A toxicity is essential for mosquito development
Fig 1
Use of ZFN results in efficient editing of the AaeCad gene.
A. A ZFN construct with zinc finger nuclease binding site (in black, uppercase) and target site (in red, lower case). B. After G1 mosquitoes were genotyped and the generated data was analyzed and plotted. Profiling of the deletion length and the mutation rate is shown for a representative group of G1 mosquitoes. C. A chromatogram of a ZFN-targeted AaeCad fragment from wild-type Orlando strain mosquitoes and heterozygous mutant with 4nt-deletion. For ZFN-targeted AaeCad fragment from the heterozygous mutant, the overlapping chromatography peaks are displayed after the 4nt deletion.
