Aedes cadherin receptor that mediates Bacillus thuringiensis Cry11A toxicity is essential for mosquito development
Fig 2
TALEN mediated knockout of the AaeCad gene.
A. In this TALEN design schematics shows AaeCad gene sequence targeted by TALEN. B. G2 mosquitoes were genotyped by native heteroduplex mobility assay (HMA). Four out of 117 mosquito groups analyzed had 3nt, 4nt, 6nt or 3nt & 4nt deletions, respectively. Lane M, DNA marker; Lane 1, sample mosquito #31M with 4nt indel; Lane 2, sample #32M with 3nt and 4nt indel; Lane 3, sample #41 with 3nt indel; Lane 4, sample #70F with 6nt indel; Lane 5, sample #24F with no indel. C. PCR product sequencing from a heterozygous mutant displayed overlapping chromatogram peaks, but those from wild-type Orlando strain mosquito showed single chromatogram peaks.
