Aedes cadherin receptor that mediates Bacillus thuringiensis Cry11A toxicity is essential for mosquito development
Fig 8
Larval exposure to low level Cry11A toxin concentrations disrupts mosquito midgut cells and causes these cells to shed its cell membrane.
The larval midgut cell morphology was observed under a confocal microscope after the mosquitos were treated at the LC10 dose for 1 hr (A), 4 hr (B), 8 hr (C) and 16 hr (D). Changes in cell conformation were observed with Armadillo staining in cadherin-EGFP mosquitoes (E, F and G) at 18h. The same image is observed with DAPI, EFGP (cadherin) and Alexa 555 (Amardillo). Significant AaeCad-EGFP and Amardillo signaling is also observed in the intracellular compartments. Bar: 50μm.
