Characterization of a dual-action adulticidal and larvicidal interfering RNA pesticide targeting the Shaker gene of multiple disease vector mosquitoes
Fig 2
siRNA-mediated silencing of Aae Sh results in neural defects in A. aegypti adults.
The high levels of Aae Sh expression detected throughout the A. aegypti adult female brain (a control siRNA-treated brain is shown in A1) were significantly reduced in adults injected with Sh.463 siRNA (A2). Adult brains were labeled with mAbnc82 (white in B1, B2; red in C1, C2), which labels synaptic active zones, and the nuclear stain TO-PRO (blue in C1, C2). nc82 levels were significantly reduced (B3) in the synaptic neuropil of adult females injected with Sh.463 siRNA (B2, C2; compare to white staining of control siRNA-injected brain in B1/red staining in C1). In A3, B3, and C3, data are represented as average mean gray values, and error bars denote SEM; *** = P<0.001 when compared with control siRNA-injected mosquitoes. Representative adult brains are oriented dorsal upward in this figure. AL: antennal lobe; OL: optic lobe; SOG: sub-esophageal ganglion; SuEG: supra-esophageal ganglion. Scale Bar = 100 μm.
