Characterization of a dual-action adulticidal and larvicidal interfering RNA pesticide targeting the Shaker gene of multiple disease vector mosquitoes
Fig 5
Sh.463 yeast induces neural defects in A. aegypti larvae.
High levels of Aae Sh expression detected throughout the A. aegypti L4 larval brain (the brain from a control yeast-treated larva is shown in A1) are significantly reduced (A3) in the L4 brain of larvae fed with Sh.463 yeast (A2). Mean gray value analysis results are displayed in A3. Larval brains were labeled with mAbnc82 (white in B1, B2; red in C1, C2) and TO-PRO (blue in C1, C2). nc82 levels were significantly reduced (B3) in the synaptic neuropil of larvae fed with Sh.463 yeast (B2, C2; compare to control-treated brain in B1, C1). In B3 and C3, data are represented as average mean gray values. In A3, B3, and C3, error bars denote SEM, and *** = P<0.001 when compared with brains from control yeast-treated larvae. Representative adult brains are oriented dorsal upward in this figure. AL: antennal lobe; OL: optic lobe; SOG: sub-esophageal ganglion; SuEG: supra-esophageal ganglion. Scale Bar = 100 μm.
