The Hippo Pathway Promotes Notch Signaling in Regulation of Cell Differentiation, Proliferation, and Oocyte Polarity
Figure 5
Defective endocytosis in Hpo pathway–mutant follicle cells.
Both NECD and NICD accumulate in hpo (A,C) and sav (B,D) PFC clones of stage 9/10 egg chambers, including ectopic cytoplasmic puncta (arrowheads). (E) In hpo follicle-cell clones (indicated by loss of GFP, false-colored blue in panel E, white in E’’), Hrs (red) accumulates at the apical region and overlaps significantly with NICD (false-colored in green to faciliate determination of colocalization by yellow signal, as shown in E'). (F) Some ectopic NICD is also found to colocalize with Rab7:GFP positive vesicles (white arrowheads) in hpo follicle cell clones (visualized by loss of lacZ in blue in panel F, white in F’’). Note in wildtype cells the Rab7:GFP-positive vesicles do not appear to contain Notch protein (red arrowheads). hpo (G) and sav (H) mutant PFC also contain discrete cytoplasmic as well as membrane-associated accumulations of Domeless protein. Staining of the endocytic marker FM4-64FX was significantly higher in hpo mutant follicle cells of stage 10 egg chambers, regardless of position in the FE (I cross-section, J top view-clone outlined in dashed yellow line).
