IL-21 Limits Peripheral Lymphocyte Numbers through T Cell Homeostatic Mechanisms
Figure 2
Reduced T cell proliferation in IL-21R-deficient mice.
Representative histogram plots for individual NOD (thin line) and IL-21R KO NOD (thick line) mice showing flow cytometric analysis of BrdU incorporation in CD4+CD44hi and CD8+CD44hi populations after 5 days of BrdU treatment. The data, obtained from analyzing the spleens of 7 to 9-week-old NOD and age-matched IL-21R KO NOD mice, is presented as mean percentages of BrdU+ cells ±SEM from one experiment with a total of n = 4 mice/group. The experiment, using the same number and age of mice, was repeated twice with similar results. (B) Representative flow cytometric analyses of splenocytes from individual C57BL/6 (thin line) and IL-21R KO C57BL/6 (thick line) mice showing BrdU incorporation in CD4+CD44hi and CD8+CD44hi T cells after 5 days of BrdU treatment. Mean percentages of BrdU+ cells ±SEM are shown from one experiment with a total of n = 4 mice/group with an average age of 10–12 weeks. The experiment was repeated three times with similar results. For both A and B, the gate on the histogram plots represents isotype control staining. (C) Representative dot plot showing expression of CD44 and CD62L in the CD4+ population of individual NOD and IL-21R KO NOD mice. Pooled data ±SEM from a total of n = 8 mice/group, at 7 to 9-weeks of age, from two independent experiments is shown. Gates are based on isotype control staining.
