IL-21 Limits Peripheral Lymphocyte Numbers through T Cell Homeostatic Mechanisms
Figure 3
Expansion of IL-21R-deficient T cells upon encounter with self-antigens.
CFSE-labeled splenocytes from 6 to 8-week-old (A) OT1 or (B) IL-21R KO OT 1 mice were adoptively transferred into wild-type Rip-OVA recipient mice, with an average age of 8-weeks, for five days. Cells were then recovered from the pancreatic lymph nodes of recipient mice and analyzed by flow cytometry for CFSE dilution to measure proliferation. A representative CFSE dilution profile of the indicated Vβ5.1+/5.2+ (donor) T cells from individual recipient mouse is shown. The data shown reflects the mean percentage of CFSEhi and CFSElo T cells ± SEM for a total of n = 15 mice/group from four independent experiments, p = 0.04. Analysis of cells before transfer indicated >80% were naïve (CD8+CD44lo, data not shown).
