Comparative Effects of Heterologous TRPV1 and TRPM8 Expression in Rat Hippocampal Neurons
Figure 2
Capsaicin-induced and capsaicin-independent currents are blocked by ruthenium red in TRPV1-transfected neurons.
(A) Examples of TRPV1-transfected (left panel) and non-transfected (right panel) neurons exposed to acute application of 500 nM capsaicin for 15 s with 10 µM ruthenium red added during the last 5 s. TRPV1-transfected neurons, but not non-transfected neurons, respond to capsaicin with a robust current that is blocked by ruthenium red. (B) Examples of TRPV1-transfected (left panel) and non-transfected (right panel) neurons exposed to acute application of 10 µM ruthenium red in the absence of capsaicin. Leaky (more than −200 pA standing inward current) TRPV1-transfected neurons reliably responded to ruthenium red while non-leaky TRPV1-transfected neurons and non-transfected neurons did not.
