Enhanced Expression of lmb Gene Encoding Laminin-Binding Protein in Streptococcus agalactiae Strains Harboring IS1548 in scpB-lmb Intergenic Region
Figure 2
Properties of 26 GBS isolates in relation to the scpB-lmb intergenic region structure (8 strains without MGE, 9 strains with GBSi1 and 9 strains with IS1548).
(A) Relative transcription levels of scpB (filled boxes) and lmb (open boxes) genes. The amount of transcripts of each gene was normalized to the amount of gyrA transcripts and expressed relative to the level of transcription in L19 GBS isolate that has no MGE. Boxes are means and bars are standard deviation of the means of the relative level of transcription in each group of strains. ** The mean transcription level of lmb gene was significantly higher in the group of strains with IS1548 than in the other two groups of strains (P<0.001). (B) Binding ability of GBS isolates to immobilized human fibronectin (filled boxes) and to immobilized human laminin (open boxes). Boxes are means and bars are standard deviation of the means of the fibronectin and laminin binding percentages in each group of strains. ** The mean binding ability to laminin was significantly higher in the group of strains with IS1548 than in the other two groups of strains (P≤0.001).
