Enhanced Expression of lmb Gene Encoding Laminin-Binding Protein in Streptococcus agalactiae Strains Harboring IS1548 in scpB-lmb Intergenic Region
Figure 3
Expression of Lmb protein on GBS cells in relation to the scpB-lmb intergenic region structure.
Lmb protein was quantified in an ELISA-type assay. GBS cells (8 strains without MGE, 9 strains with GBSi1, 9 strains with IS1548), were immobilized in microtiter wells and incubated with rabbit anti-Lmb antibodies. Binding of antibodies to the Lmb protein was quantified by addition of peroxidase-conjugated goat anti-rabbit IgG, and subsequent addition of OPD peroxidase substrate. Boxes are means and bars are standard deviation of the means of the absorbance at 492 nm (A492) per adhering bacteria in each group of strains. ** The amount of Lmb protein was significantly higher in the group of strains with IS1548 as compared with the other two groups of strains (P<0.001).
