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Enhanced Expression of lmb Gene Encoding Laminin-Binding Protein in Streptococcus agalactiae Strains Harboring IS1548 in scpB-lmb Intergenic Region

Figure 4

Properties of L29 wild type GBS strain and isogenic IS1548 deletion mutant.

Relative transcription levels of lmb gene, binding ability of GBS cells to immobilized human laminin, and expression of Lmb protein on GBS cells as determined by ELISA, in an isogenic mutant deleted for IS1548 sequence between scpB and lmb genes (L29Ī”IS1548) (striped boxes) as compared to the parent strain (L29 WT) (open boxes). Each experiment was performed at least three times. Boxes are means and bars are standard deviation of the means.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0010794.g004