Hypoxia-Inducible Factor 1α Determines Gastric Cancer Chemosensitivity via Modulation of p53 and NF-κB
Figure 1
HIF-1α mediates resistance towards the chemotherapeutic agent 5-FU in AGS cells.
(A) Proliferation of AGS KD and SCR cells 24 h after treatment with increasing concentrations of 5-FU under normoxic conditions. Cell numbers are shown as percent of untreated cells (*, P<0.05; **, P<0.01). (B) AGS wild-type cells were transfected with HIF-1α expression vector (pcDNA HIF-1α) or empty vector (pcDNA 3.1) and treated with 5-FU 24 h post transfection. Cell numbers were determined 24 h after treatment with 5-FU, and are shown as percent of untreated control cells (**, P<0.01). (C) AGS wild-type cells were co-transfected with either pcDNA HIF-1α or pcDNA 3.1 plus HRE-Luciferase reporter (pHRE-Luc) and Renilla reporter (phRL-null) as internal control. Cells were harvested 48 h post transfection. HRE luciferase activity, normalised for Renilla luciferase activity, was expressed relative to that of control transfected cells (***, P<0.001).
