Hypoxia-Inducible Factor 1α Determines Gastric Cancer Chemosensitivity via Modulation of p53 and NF-κB
Figure 3
siRNA silencing of p53 reverses the chemosensitization of AGS KD cells.
AGS cells were transfected with control siRNA (si scr) or siRNA against p53 (si p53), and 10 µg/ml 5-FU was added 24 h post transfection. (A) Immunoblot analysis for p53, p21 and MDM2 in whole cell extracts from AGS KD cells after 5-FU treatment. Actin served as loading control. (B and C) Cell numbers of si scr and si p53 transfected AGS cells was determined 24 h after treatment with 5-FU. Results are shown as percent of untreated control cells (**, P<0.01). Cells in the G1 phase (D) and thesub-G1 population (E) were evaluated from DNA histograms of AGS KD cells transfected with si p53 or si scr and treated for 24 h with 5-FU (*, P<0.05; **, P<0.01).
