Hypoxia-Inducible Factor 1α Determines Gastric Cancer Chemosensitivity via Modulation of p53 and NF-κB
Figure 4
Effects of 5-FU on MKN28 cells with mutant p53.
(A) Cell numbers of MKN28 KD and SCR cells 24 h after treatment with 5-FU under normoxic conditions. Data are shown as percent of untreated cells. Cells in G1 phase (B) and apoptotic cells (C) were quantitated from DNA histograms of MKN28 KD and SCR cells treated for 48 h with 10 µg/ml 5-FU. (D) Immunoblot analysis of p53, p21 and pRb protein levels in MKN28 KD and SCR cells treated with 10 µg/ml 5-FU for 6 and 24 h. Actin served as loading control.
