Akt Deficiency Attenuates Muscle Size and Function but Not the Response to ActRIIB Inhibition
Figure 5
Effects of Akt deficiency and ActRIIB inhibition on soleus fiber size and distribution.
(A) Effects of genotype and ActRIIB-mFc treatment on soleus type I and type II fiber cross-sectional area distributions. Wild-type (WT, red), Akt1 knockout mice (Akt1 KO, green), Akt2 knockout mice (Akt2 KO, blue), ActRIIB-mFc -treated WT (WT Rx, red-dashed), ActRIIB-mFc -treated Akt1 KO (Akt1 KO Rx, green dashed), and ActRIIB-mFc -treated Akt2 KO (Akt2 KO Rx, blue dashed) on soleus type I fiber distribution; n≥393 fibers per group. (B) Type I single fiber cross sectional area (CSA) per genotype (Veh, white bar) and treatment (Rx, black bar); (C) Number of type I fibers in the soleus; (D) Soleus type II fiber distribution; n≥458 fibers per group. (E) Type II single fiber CSA per genotype and treatment; (F) Number of type II fibers in the soleus; Data are mean +/− SEM, n = 5; +P<0.05 vs. WT treated with vehicle; *P<0.05 vs. same genotype treated with vehicle.
