HelmCoP: An Online Resource for Helminth Functional Genomics and Drug and Vaccine Targets Prioritization
Figure 4
Properties of dihydrofolate reductase, a putative drug target.
(A). Alignment of the secondary-structure predictions downloaded from HelmCoP using JalView (Waterhouse et al. 2009) for the following sequences: 15204.m00021 (B. malayi), Mh10g200708_Contig1584_9859_10502 (M. hapla), Sjc_0071810 (S. japonica), Smp_175230 (S. mansoni), prot_Minc17846 (M. incognita), 3D84 (PDB entry). The sequences are colored based on clustalw (Thompson et al. 1994). For the secondary-structure prediction, helices are colored red, β-sheets blue, and random coils and loops are colored yellow. The fasta from the PDB entry, 3D84, is also shown at the bottom of the alignment. Secondary structure was aligned using a script (provided in the supp information) after aligning fasta sequences provided by HelmCoP using MUSCLE (Edgar 2004). The fasta sequence for the PDB entry was obtained from the PDB website. B. PDB entry 3D84 rendered using Pymol (Version 1.2r3pre) with helices, loops, and β-sheets colored red, yellow, and blue, respectively C. Chemical structures of DrugBank compounds that bind to dihydrofolate reductase targets, which have also been used as antimalarial drugs.
