Evaluation of Pulsed-FRAP and Conventional-FRAP for Determination of Protein Mobility in Prokaryotic Cells
Figure 2
The ”conventional” FRAP method.
A–E: snapshots of a cell during data acquisition; A: the cell before photobleaching (panel labeled “pre”); B–E: the cell during recovery after photobleaching (timestamp indicates the time after the photobleaching pulse). Dotted circle indicates the bleaching spot (B). Scale bar 2 µm. F–J: the fluorescence intensity along the dotted cross-section at given time points (corresponding to images on the left: A–E), where black is the normalized fluorescence intensity and red is the fit. K,L: the change of intensity in the course of the recovery at (K) the bleached pole of the cell (R1) and at (L) the opposite pole of the cell (R2). Black squares show the normalized data points and red lines the corresponding fits.
