LPS Regulates SOCS2 Transcription in a Type I Interferon Dependent Autocrine-Paracrine Loop
Figure 1
Various TLR ligands induce SOCS2 gene expression in human moDCs.
(A). Human SOCS2 mRNA expression induced by different TLR signaling. Enriched monocytes were cultured in the presence of GM-CSF and IL-4 for 6 days. Quantitative real-time PCR was used to measure SOCS2 mRNA expression in iDC after exposure to Pam3CSK4 (TLR1/2), LPS (TLR4), flagellin (TLR5), polyI:C (TLR3), imiquimod (TLR7), ssRNA40 (TLR8) and ODN 2336 (TLR9) for different time periods. Data shown are the mean of triplicate determinations from at least three donors. Values for the time point 0 were set to 1. (B, C). SOCS2 protein expression levels after various TLR signaling stimuli. Western Blotting analysis for protein of SOCS2 expression in iDCs exposed to Pam3CSK4 (TLR1/2), LPS (TLR4), flagellin (TLR5), polyI:C (TLR3), imiquimod (TLR7), ssRNA40 (TLR8) and ODN 2336 (TLR9) for different time points. (B) Representative Western Blot for each typical TLR signaling effect to SOCS2 expression. The data shown in (C) are the mean of SOCS2 protein bands quantified from Western blots and normalized to β-actin from four donors. Values for time point 0 were set to 1. Error bars comparing different time point groups to the 0 time point group illustrate s.d. * p<0.05; ** p<0.01.
