LPS Regulates SOCS2 Transcription in a Type I Interferon Dependent Autocrine-Paracrine Loop
Figure 4
STAT3 and STAT5, but not STAT6 are required for SOCS2 mRNA induction in human moDCs.
(A) STAT3 and STAT5, but not STAT6 were translocated into the nucleus after IFNβ or LPS stimulation. moDCs were incubated with IFNβ or LPS at the indicated time points. The cells were then harvested and nuclear proteins were extracted for Western Blotting measurement. The levels of STAT3, STAT5, STAT6 and LaminA (as loading control) were detected. (B, C and D) STAT3 and STAT5, but not STAT6 gene silencing eliminates SOCS2 mRNA induction in TLR4 signaling. moDCs were transfected with (B) STAT3, (C) STAT5 or (D) STAT6 siRNAs or control siRNA and incubated for 24 hours. The transfected cells were exposed to LPS for 0 h, 8 h and 24 h, the cells were then harvested for the mRNA measurement by qRT-PCR to demonstrate the effect of SOCS2 mRNA induction (left) and efficiency of target gene silencing (right). Data shown are representative of three independent experiments and expressed as the –fold induction of the gene of interest at different time points compared to time point 0 for control siRNA where values were set to 1. Significance comparing results from the target siRNA to the control siRNA group is indicated (* p<0.05; ** p<0.01).
