Selective Induction of Cell Death in Melanoma Cell Lines through Targeting of Mcl-1 and A1
Figure 3
Melanoma-specific cell death can be enhanced by combined inhibition of Mcl-1 and A1.
(A) Fibroblasts (left panel) and 1205Lu melanoma cells (right panel) were transfected with the indicated siRNAs for 48 hours. Thereafter, 1 µM ABT-737 was added and cell death was assessed 24 hours after ABT-737 treatment. (B) Fibroblasts were simultaneously transfected with two siRNAs as indicated. 48 hours after transfection the respective protein was analyzed by immunoblotting (upper panels) or RT-PCR (lower panel). Blots are representative for 3 (upper left panel) or 2 (upper right panel) independent experiments. Cell death analysis of (C) fibroblasts or (D) 1205Lu melanoma cells treated with the indicated siRNAs for 72 hours. Mean +/− SD of 3 independent experiments is shown in A, B, C and D. α-tubulin or β-actin served as loading control in immunoblots. White lines indicate lanes that were run at the same blot but are not contiguous. Asterisks represent significant increase in cell death compared to control siRNA-treated cells. AN, Annexin V; PI, propidium iodide.
