Identification and Validation of Ifit1 as an Important Innate Immune Bottleneck
Figure 2
Silencing Ifit1 suppresses LPS activation of Usp18 and M×1.
siRNA against Ifit1 or a negative control were introduced into RAW264.7 macrophages by transfections and the macrophages were treated with 1 ng/mL of LPS. Expression of Ifit1 (A), Usp18 (B) and M×1 (C) were measured by RT-PCR at 3 hours post-LPS treatment. The results show that Ifit1 exerts a positive regulatory effect on Usp18 and M×1.
