Synapse Associated Protein 102 (SAP102) Binds the C-Terminal Part of the Scaffolding Protein Neurobeachin
Figure 2
Nbea interacts with SAP102 in HEK293T cells.
Co-imunoprecipitation of Nbea and SAP102. (A) HEK 293T cells were co-transfected with full-length Nbea tagged with YFP and flag-tagged SAP102 or an empty vector and were immunoprecipitated (IP) with α-GFP antibody before immuno-blotting (IB) with α-Nbea and α-flag antibody. In the control condition non-coated, empty beads (EB) were used for the IP. (B) Reverse IP to the ones in A. HEK 293T cells were co-transfected with full-length Nbea tagged with YFP and flag-tagged SAP102 or an empty vector, but this time they were immuno-precipitated (IP) with α-flag antibody before immuno-blotting (IB) with α-Nbea and α-flag antibody. In the control condition non-coated, empty beads (EB) were used for the IP.
