Synapse Associated Protein 102 (SAP102) Binds the C-Terminal Part of the Scaffolding Protein Neurobeachin
Figure 3
SAP102 binds to the C-terminal part of Nbea.
(A) HEK293 cells were co-transfected with flag-tagged SAP102 and either full-length Nbea tagged with YFP or various Nbea deletions encompassing different domains fused to GFP. Following deletions were used: GFP-Duf, PH, BEACH, WD40; GFP-Duf, PH, BEACH; GFP-PH, BEACH, WD40; GFP-PH, BEACH; GFP-PH; GFP-BEACH. In the control condition SAP-102 was co-transfected with YFP and GFP. IPs were performed using the α-GFP antibody, before immune-blotting with α-flag and α-GFP antibodies. (B) Quantification of flag and GFP protein levels using the immuno-labelled bands. Error bars indicate the standard error of the mean (SEM).
