Neisseria gonorrhoeae Suppresses Dendritic Cell-Induced, Antigen-Dependent CD4 T Cell Proliferation
Figure 6
N. gonorrhoeae inhibits dendritic cell-induced T cell proliferation in human primary immune cells.
A) Representative histograms from 2 donors showing unregulated expression of CD11c, HLA-DR, CD274 and CD273 at 24 hours post stimulation with N. gonorrhoeae (MOI = 1, 10). B) IL-10 protein production by human DCs treated with N. gonorrhoeae (MOI = 1,10). C) MFI of PD-L1 expression on human DCs treated with N. gonorrhoeae (MOI = 1,10). D) N. gonorrhoeae inhibits human DCs induced allogeneic T cell proliferation in the Mixed Lymphocyte Reaction (MLR). CFSE proliferation profiles of CD4+ cells after non-adherent cells (NAD) co-cultured with human DCs treated with medium or N. gonorrhoeae (MOI = 10) for 7 days at the ratio of 10∶1.
