Ubiquitination and Degradation of the Hominoid-Specific Oncoprotein TBC1D3 Is Mediated by CUL7 E3 Ligase
Figure 4
CUL7 siRNA silencing delays TBC1D3 degradation.
(A) HeLa cells were transfected with scramble siRNA or CUL7 siRNA. At 24 h post-transfection, Myc-TBC1D3 was expressed in the cells for another 18 h. The cells were then starved for 3 h and stimulated with 10% FCS for different time periods. Cell lysates were prepared, separated by SDS-PAGE and immunoblotted with anti- TBC1D3, CUL7 and tubulin antibodies. (B) Summary of results. Mean ± SE of 3 independent experiments. TBC1D3 expression is normalized by tubulin. The initial level of TBC1D3 expression in each group is set to 100%.
