Effects of Exposure to a DNA Damaging Agent on the Hypoxia Inducible Factors in Organogenesis Stage Mouse Limbs
Figure 1
HIF1 heterodimer DNA binding activity in limbs exposed to 4-OOHCPA or vehicle.
Limbs in culture were treated with saline (control) or 4-OOHCPA (1 µg/ml or 3 µg/ml) and collected after 30 min, 1, 3 or 6 h. HIF1 DNA binding activity was measured using an ELISA assay as described in the Methods. The data are expressed as mean ± SEM (µg nuclear extract standard/µg sample protein); each bar represents 3–4 litters. * indicates a significant difference from control at that time point (P<0.05).
