Effects of Exposure to a DNA Damaging Agent on the Hypoxia Inducible Factors in Organogenesis Stage Mouse Limbs
Figure 3
4-OOHCPA exposure induced HIF2A.
A: Western blot analysis of HIF2A protein (118KD) and actin (43KD) in limbs at 10 min, 1, 3, 6, and 24 h after treatment with 4-OOHCPA at 0.3 µg/ml (L) 1.0 µg/ml (M) or 3.0 µg/ml (H). P represents the positive control. B: Fold changes from the scan densitometry quantification of the HIF2A band in drug treated groups compared to control groups. Hatched bar: 0.3 µg/ml (L), cross-hatched bar: 1.0 µg/ml (M) and gray bar: 3.0 µg/ml (H). Each bar (mean ± SEM) represents six to seven replicates; bars with an asterisk are significantly different from control at the same time point (p ≤0.05).
