Effects of Exposure to a DNA Damaging Agent on the Hypoxia Inducible Factors in Organogenesis Stage Mouse Limbs
Figure 5
HIF3A protein was detected in control limbs at all time points. A: Western blot analysis of HIF3A protein (118 KD) and actin (43 KD) in limbs at 30 min, 1, 3, 6, and 24 h after treatment with 4-OOHCPA at 0.3 µg/ml (L) 1.0 µg/ml (M) or 3.0 µg/ml (H). B: Densitometric quantification of HIF3A in drug treated groups compared to control groups. Hatched bar: 0.3 µg/ml (L), cross-hatched bar: 1.0 µg/ml (M) and gray bar: 3.0 µg/ml (H). HIF3A protein concentrations were not affected by drug treatment or time. Each bar (mean ± SEM) represents six to seven replicates.
