Conditional Knockout of Integrin α2β1 in Murine Megakaryocytes Leads to Reduced Mean Platelet Volume
Figure 5
Platelet function: in vitro adhesion to soluble type I human collagen.
Washed platelets from f/f, f/− and −/− mice were allowed to adhere under static conditions to soluble type I collagen immobilized on microtiter plates. Adherent platelets were quantitated by measuring Absorbance at 405 nm (ordinate) in the absence of inhibitory antibody at 15, 30, 45 and 60 minutes (abscissa) or in the presence of the GPVI inhibitory antibody JAQ1 at 60 minutes. The results of a single experiment, representative of three identical experiments, are depicted as the mean ± SD. The asterisks (*) denote results that are significantly different (p<0.5) from those obtained for f/f mice.
