Mouse Model of Lymph Node Metastasis via Afferent Lymphatic Vessels for Development of Imaging Modalities
Figure 2
Establishment of the model of lymph node metastasis.
A. Representative images captured by in vivo bioluminescence imaging of a mouse with tumor cells grafted into the SiLN to promote metastasis to the ALN. (a) In vivo and (b) ex vivo bioluminescence signals in the proper-ALN and SiLN on day 14 post-inoculation, indicating that the proper-ALN is the draining lymph node. B. Graph showing the high correlation between in vivo and ex vivo bioluminescence (P = 0.0023; Spearman’s rank correlation coefficient [rs] = 0.9161; SiLN, n = 6; proper-ALN, n = 6). C. Results of histological verification. Tumor cells stained with H&E and luciferase-positive immunohistochemical signals in the proper-ALN and SiLN. MS: marginal sinus. T: tumor. D. Dissemination of KM-Luc/GFP cells (metastasis, n = 4) or PBS alone (control, n = 3) to each organ, assessed on day 14 post-injection of the SiLN. I, ipsilateral; C, contralateral; LN, lymph node. Error bars indicate the SEM values.
