Selection of Appropriate Reference Genes for RT-qPCR Analysis in a Streptozotocin-Induced Alzheimer’s Disease Model of Cynomolgus Monkeys (Macaca fascicularis)
Figure 1
GeNorm analysis of the total sample, control, and icv-STZ groups.
Average expression stability (M) of 9 candidate reference genes and the best combination of 2 genes were calculated (A, C, and D). Lower M values indicate more stable expression. Determination of the optimal number of reference genes for normalization was conducted (B, D, and F). The geNorm program calculated the normalization factor (NF) from at least 2 genes and the variable V defines the pair-wise variation between the 2 sequential NF.
