Notch1 Is a 5-Fluorouracil Resistant and Poor Survival Marker in Human Esophagus Squamous Cell Carcinomas
Figure 1
Quantitative RT-PCR of Notch family in the squamous esophageal cancer cell lines KYSE70, KYSE140 and KYSE450 and the virus transformed normal squamous esophageal epithelial cell line Het-1A (A).
Each PCR was performed twice with almost identical values. Notch1 protein expression was examined by Western blotting (B), showing strong Notch1 in KYSE70 cells, negative in KYSE450 cells and weak positive in both KYSE140 and Het-1A cells. Conventional RT-PCR shows rather the same intensity PCR bands for both KYSE70 and KYSE450 cells with the two primer pairs (C).
