microRNA-122 Dependent Binding of Ago2 Protein to Hepatitis C Virus RNA Is Associated with Enhanced RNA Stability and Translation Stimulation
Figure 5
Length variation of the miR-122 duplex precursor impairs stimulation of HCV translation.
(A) Structure of the miR-122 duplex (22 nucleotides) and its length variants. The base-pairing conditions at the thermodynamically less stable end (left side) and the 2 nucleotide 3′-overhangs at both ends were retained in all cases. (B) Stimulation of HCV reporter RNA translation in HeLa cells after co-transfection of the RNA duplexes shown in (A) as indicated. miR-124 was used as negative control, and its readout was set to 100%.
