microRNA-122 Dependent Binding of Ago2 Protein to Hepatitis C Virus RNA Is Associated with Enhanced RNA Stability and Translation Stimulation
Figure 7
The HCV reporter RNA and the stem insertion mutant (st, see Fig. 6B) were transfected into HuH-7 cells. Additional ectopic miR-122 (or miR-124 as a control) was added as indicated. After the times indicated, the cells were lysed and Fluc activity determined. (A) Display of the Fluc activities with all readouts normalized to the wt HCV RNA in the presence of miR-122 after 3 h. All other readouts were normalized to this value to display the stimulation of translation by miR-122 at the 3 h time point and later time points. Error bars indicate standard deviations from four independent experiments. (B) The same readouts as shown in (A) but with the values obtained from expression of all combinations of HCV reporter RNAs and microRNAs at 3 h after transfection normalized to 100%. In this diagram, the differential decay in the presence of miR-122 or miR-124, respectively, is clearly obvious. * = p<0.05; ** = p<0.01.
