Global and Stage Specific Patterns of Krüppel-Associated-Box Zinc Finger Protein Gene Expression in Murine Early Embryonic Cells
Figure 1
Mouse and human gene families encoding for KRAB-containing proteins and their conservation.
A) Summary table of the mouse and human families. B) Venn diagram representing the comparison between mouse (left panel) and human (right panel) KRAB-ZFP gene lists generated in this work and in previously published studies [1], [5], [42], [48]. Numbers indicates the KRAB-ZFP genes identified in the different studies and the overlap among the different lists. C) Alignment of the C2H2 ZF domain amino acid sequences (excluding spacer sequences) of three mouse and human KRAB-ZFPs generated with Clustal Omega. Dark purple boxes indicate conserved residues. Light purple boxes indicate non-conserved residues. Dashes indicate gaps introduced by the alignment tool. Red residues highlighted in the consensus sequence point to the conserved cysteine and histidine residues of each C2H2 ZF domain. Human ZNF746 and mouse ZNF746 show 100% homology at the level of their C2H2 ZF domains. Human RBAK and mouse ZFP12 show 74.1% homology between their C2H2 ZF domains. Human ZFP57 and mouse ZFP57 show only 30.5% global homology between their C2H2 ZF domains, but the human third and fourth C2H2 ZF domains are homologous to the mouse first and second C2H2 ZF domains that are necessary and sufficient for the recognition of the conserved target sequence contained in ICRs [39].
