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An Isoform of Eukaryotic Initiation Factor 4E from Chrysanthemum morifolium Interacts with Chrysanthemum Virus B Coat Protein

Figure 4

Interaction between CmeIF(iso)4E and CVBCP in vitro as demonstrated by Y2H assay.

Yeast two-hybrid screen demonstrating the interaction between CVBCP and CmeIF(iso)4E. The left panel shows the growth of yeast cells containing both plasmids on SD-Leu-Trp medium (SD-2); the middle panel shows the selection of yeast colonies on SD/-Leu/-Trp/-Ade/-His medium (SD-4); the right panel shows the selection of yeast colonies on SD-4 containing α- X-Gal.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0057229.g004