Overexpression of CD44 in Neural Precursor Cells Improves Trans- Endothelial Migration and Facilitates Their Invasion of Perivascular Tissues In Vivo
Figure 1
Effective CD44 lentiviral transduction of NPCs :
(A) FACS analysis and (B) immunoblot of CD44-c-myc transduced cells with an antibody revealing CD44-c-myc and endogenous cm-myc showing c-myc (and thus CD44) overexpression in transduced cells. (C) Quantitative data indicating that lentiviral transduction does not change the cell phenotype. (D–I) Representative illustrations of actin eGFP cells (green) expressing CD44 (red), nestin (red) and Ki67 (red) in response to EGF and FGF (D;E;F: control NPCs; G;H;I: CD44-NPCs). (J–L) Transduced cells represent nearly 70% of the cell population; immunocytochemistry for c-myc (red) in actin eGFP (green) control-(K) and CD44-NPCs (L).
