Overexpression of CD44 in Neural Precursor Cells Improves Trans- Endothelial Migration and Facilitates Their Invasion of Perivascular Tissues In Vivo
Figure 7
Correlation of GFP expressing cells and CD45 expressing cells in splenic ganglia (AāD) and in the tail vein (EāH) 6 h after i.v delivery.
(AāC) Immunohistochemistry for CD45 (red) illustrating the presence of GFP positive cells (arrow) at proximity of CD45+ cells in the ganglia. (D) The presence of GFP transduced cells in the ganglia is confirmed by PCR for eGFP. (EāH): Close association of CD44-NPCs with CD45+ cells during trans-endothelial migration at the site of injection. Several GFP expressing cells are in close contact with CD45+ cells in the vascular wall. Arrow in E, point to the region enlarged in F, G, H.
