Novel Binding Partners and Differentially Regulated Phosphorylation Sites Clarify Eps8 as a Multi-Functional Adaptor
Figure 5
Full-length Eps8 interacts with a range of proteins identified in the peptide pull down assays.
HEK 293T cells were either transfected with Myc-Eps8 or left untransfected. Cells were stimulated with 20 ng/ml FGF2 for 15 min and immunoprecipitated using an anti-Myc antibody. Western blot analysis was carried out on whole cell lysate and immunoprecipitation samples using antibodies against the indicated proteins.
