Novel Binding Partners and Differentially Regulated Phosphorylation Sites Clarify Eps8 as a Multi-Functional Adaptor
Figure 7
Eps8 and IRS4 colocalise within cells in an FGF2 dependent manner.
NIH 3T3 cells were co-transfected with IRS4-GFP and Eps8-mCherry. Cells stimulated with FGF2 (20 ng/ml) in the presence and absence of SU5402 (25 µM) were compared to unstimulated cells (control). A) Confocal microscopy was used to visualise the localisation of IRS4 and Eps8. B) The colocalisation (Pearson’s coefficient) between IRS4 and Eps8 is significantly increased in the presence of FGF2 and absence of SU5402 (Pearson’s coefficient, mean±SEM, n = 42 cells. Scale bars = 5 µm. ***, P<0.001).
