Identification and Characterization of Pheromone Receptors and Interplay between Receptors and Pheromone Binding Proteins in the Diamondback Moth, Plutella xyllostella
Figure 4
Responses of Xenopus oocytes with co-expressed PxylOR1/PxylOrco or PxylOR4/PxylOrco to stimulation with pheromone compounds.
(A) (Upper left) Inward current responses of PxylOR1/PxylOrco Xenopus oocytes in response to 10−4 M of pheromone compounds and analogs. (Upper right) Response profile of PxylOR1/PxylOrco Xenopus oocytes. Error bars indicate SEM (n = 6). (Lower left) PxylOR1/PxylOrco Xenopus oocytes stimulated with a range of Z11-16: Ald concentrations. (Lower right) Dose–response curve of PxylOR1/PxylOrco Xenopus oocytes to Z11-16:Ald. Responses are normalized by defining the maximal response as 100%. Z11-16: Ald EC50 = 2.39×10−5 (n = 5). Error bar indicates SEM. (B) (Upper left) Inward current responses of PxylOR4/PxylOrco Xenopus oocytes in response to 10−4 M of pheromone compounds and analogs. (Upper right) Response profile of PxylOR4/PxylOrco Xenopus oocytes. Error bars indicate SEM (n = 7). (Lower left) PxylOR4/PxylOrco Xenopus oocytes stimulated with a range of pheromone Z9-14: Ac and analog Z9, E12-14: Ac concentrations, respectively. (Lower right) Dose–response curve of PxylOR4/PxylOrco Xenopus oocytes to Z9-14: Ac and Z9, E12-14: Ac. Responses are normalized by defining the maximal response as 100%. Z9-14: Ac EC50 = 2.43×10−7 (n = 7) and Z9, E12-14: Ac EC50 = 1.94×10−6 (n = 5). Error bar indicates SEM.
