Identification and Characterization of Pheromone Receptors and Interplay between Receptors and Pheromone Binding Proteins in the Diamondback Moth, Plutella xyllostella
Figure 6
PBP-mediated responses of PxylOR1/PxylOrco Xenopus oocytes.
(A) Inward current responses of PxylOR1/PxylOrco Xenopus oocytes in response to 10−5 M of Z11-16: Ald solubilized by DMSO, 1XRinger, or each of three PxylPBPs, respectively. (B) Response profile of PxylOR1/PxylOrco Xenopus oocytes. Error bars indicate SEM (n = 5). Statistical comparison of responses of oocytes was assessed using one-way analysis of variance (ANOVA). (C) Dose–response profile of PxylOR1/PxylOR2Xenopus oocytes upon stimulation with different Z11-16: Ald concentrations solubilized by DMSO (n = 5), 1 µM PxylPBP1 (n = 4) and 1 µM PxylPBP3 (n = 4), respectively. Responses are normalized by defining the maximal response as 100%. Error bar indicates SEM.