Identification and Characterization of Pheromone Receptors and Interplay between Receptors and Pheromone Binding Proteins in the Diamondback Moth, Plutella xyllostella
Figure 7
PBP-mediated responses of PxylOR4/PxylOrco Xenopus oocytes to Z9-14: Ac.
(A) Inward current responses of PxylOR4/PxylOrco Xenopus oocytes in response to 10−5 M of Z9-14: Ac solubilized by 1XRinger, or each of three PxylPBPs, respectively. (B) Response profile of PxylOR4/PxylOrco Xenopus oocytes. Error bars indicate SEM (n = 5). Statistical comparison of responses of oocytes was assessed using one-way analysis of variance (ANOVA). (C) Dose–response profile of PxylOR4/PxylOrco Xenopus oocytes upon stimulation with different Z9-14: Ac concentrations solubilized by 1XRinger (n = 7), 1 µM PxylPBP1 (n = 4), 1 µM PxylPBP2 (n = 4), 1 µM PxylPBP3 (n = 4), respectively. Responses are normalized by defining the maximal response as 100%. Error bar indicates SEM.
