Nicotine Inhibits Memory CTL Programming
Figure 3
Nicotine reduces CTL expansion after transfer into recipients.
Purified OT-I cells were cultured for 3 days with 3SI in the presence of nicotine at different concentrations. Three days after stimulation, CTLs were harvested and transferred into B6 recipients at 106 cells/mouse. A–B. Comparison of percentage of OT-I cells in total CD8 cells in blood at day 5 (A) and 14 (B) post transfer. C–D. Comparison of the phenotype of OT1 cells in blood from mice at day 5 (C) and 14 (D) post transfer. Data are obtained from one experiment with 10 to 11 mice per group. Asterisks indicate statistical significance. *, P<0.05; **, P<0.01; ***, P<0.001.
