Atg4b-Dependent Autophagic Flux Alleviates Huntington’s Disease Progression
Figure 7
AZD8055 reduces mHtt levels in (CAG)150-het slices.
A) Single confocal planes for the immunohistochemistry of (CAG)150-het slices against mHtt (green), DARPP-32 (red), DAPI (blue) and treated with AZD8055 or vehicle at DIV21. Note, how AZD8055 treatment leads to a reduction of striatal mHtt accumulation in (CAG)150-het slices. B) Left: higher magnification of MSNs in (CAG)150-het slices visualized with DAPI (blue), DARPP-32 (red) and mHtt (green) at DIV21. Right: quantification of mHtt mean intensity. AZD8055 leads to a significant reduction of mHtt intensity. C) Western blot analysis of (CAG)150-het slices treated with AZD8055 also show a significant reduction in mHtt levels which is quantified on the right. N = 10 images from 5 independent slices; median values ± SEM; Student’s t test: *p<0.05 Bars: (A) 20 µm, (B) 10 µm.
