Accessibility of Myofilament Cysteines and Effects on ATPase Depend on the Activation State during Exposure to Oxidants
Figure 5
Confirmation of accessible cysteines in fast skeletal muscle.
A. 2D NEPHGE gel (pH 3–10) of rat EDL myofibrils with the TMR (red) and Sypro (green) scans superimposed. Yellow indicates overlap of TMR and Sypro fluorescence signals. B. TMR (left, red) and Sypro (right, green) scans from myofibrils (EDL top, Ventricles bottom) labeled in TMR then run in SDS-PAGE gels in sample buffer without added calcium (lane 1) or with 1 mM added calcium (lane 2).
