Accessibility of Myofilament Cysteines and Effects on ATPase Depend on the Activation State during Exposure to Oxidants
Figure 6
Direct comparisons between cysteine accessibilities in myofilament proteins from different muscles.
A. SDS-PAGE gels of purified rabbit myosin heavy chain and light chains (MHC, RLC, MLC1, MLC3) and rat EDL myofibrils, treated with TMR and run in adjacent lanes. TMR and Sypro scans (left and right) indicate that cysteines in fRLC, fMLC1 and fMLC3 are accessible, but cysteines in fTnC are not. B,C. SDS-PAGE gel shows the Sypro and TMR scans of fast (rat EDL), slow (rat soleus), and cardiac muscle (rat ventricle), showing accessibility of the low molecular weight proteins from each fiber type.
