Accessibility of Myofilament Cysteines and Effects on ATPase Depend on the Activation State during Exposure to Oxidants
Figure 7
Ventricular and EDL myofibrils contain a population of cysteines that are inaccessible to oxidants.
TMR and Sypro scans from (A) EDL and (B) ventricular myofibrils treated with TMR in either rigor buffer or 8 M urea buffer to compare the accessibility of cysteines in the myofilament lattice to accessibility in denatured proteins. The ratio of fluorescence intensities in rigor to urea buffers for each myofilament protein is plotted on the right (mean ± SEM, n = 5). Red stars indicate that the TMR fluorescence intensities under the two treatments were significantly different (p<0.05).
